Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System
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چکیده
منابع مشابه
Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane pro...
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متن کاملYeast two-hybrid system
Introduction The two-hybrid system (THS), first introduced by Fields and Song (1989) (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest. Here, we present not a review of the current THS technology but, rather, a comprehe...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2003
ISSN: 1088-9051
DOI: 10.1101/gr.1276503